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National Curriculum Assessment: Key Stage 2 English Language Literacy: key concepts and analysis of key concepts
National Curriculum Assessment: Key Stage 2 English Language Literacy: Analysis of texts
Category:English language tests
Category:Standardized tests
Category:Education in England
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Category:School examinations in the United Kingdom
Category:English-language educationTranscriptional activation of the human osteocalcin gene by a human tissue-nonspecific alkaline phosphatase-derived peptide.
The development of antiresorptive agents for osteoporosis has been hampered by the lack of an appropriate model system for bone biology. We have developed a quantitative assay for measuring transcriptional activation of the human osteocalcin (OC) gene. This assay was used to screen candidate factors for OC promoter activation. We found that bone-derived human tissue-nonspecific alkaline phosphatase (TNAP) induced transcriptional activation of the OC gene in a cell-free assay using HeLa nuclear extracts. Since TNAP is a major membrane-bound bone-derived ectoenzyme and produces a peptide that shares sequence homology with the amino-terminus of human OC, we synthesized a peptide corresponding to the amino-terminus of OC (POC) and tested its ability to activate the OC promoter in the HeLa nuclear extract-based OC gene transcriptional activation assay. Although the synthetic POC peptide alone did not activate the OC promoter, it activated the OC promoter when co-incubated with TNAP. The effect was concentration-dependent and was blocked by a specific POC-derived OC-cDNA. These data indicate that the POC peptide can substitute for the action of TNAP, and suggest that the endogenous POC peptide is a physiologically relevant regulator of OC gene transcription.Filling or packing a container is a process in which a product, a filler, is packed into a container in which the product does not exist (hereinafter referred to as an empty container) or a container in which the product is already existing (hereinafter referred to as an existing container) until the container is full.
In recent years, the production of containers has been actively studied in order to protect the environment. There is a need for reducing the usage of an existing container and increasing the usage of an empty container. Particularly, in the case of resin containers, the resin for producing a container is
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